Figure 9.
Effect of HGF on the invasive ability of PC-14, A549 and Lu-99 cells. PC-14, A549 or Lu-99 cells (1x105/chamber) were seeded onto filters precoated with 5 µg of Matrigel on the upper surface and 1 µg of fibronectin on the lower surface in Transwell chambers in the presence or absence of HGF (10 ng/ml). After 6 hours of incubation, the number of cells that had invaded to the lower surface was determined by crystal violet staining and colorimetric assessment by measuring the absorbance at 590 nm. *P < .05, as compared with control by Student's two-tailed t-test.