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The recombinant FLAG–hCLS1 protein transiently expressed in COS-7 cells was resolved on SDS/PAGE and analysed by Western-blot analysis using anti-FLAG antibodies. The recombinant hCLS1 exhibited two moieties, with molecular masses of 32 and 27 kDa respectively. The 32 kDa band represents the full-length hCLS1 protein, whereas the 27 kDa band represents a proteolytic degradation product from the C-terminus.
