FIG. 9.

Effect of overexpressing TSG101 and derivatives on uptake of EGF and localization of EGF-R. (A) HeLa cells were transfected with the TSG-F expression vector. Cells were incubated with 0.4 μg/ml EGF-Tx for 30 min at 37°C, fixed, permeabilized with methanol, and stained for TSG using anti-HA antiserum. (B to D) The cells transfected with TSG-F, TSG-5′ and TSG-3′ expression vectors were stimulated using EGF (0.4 μg/ml) for 30 min at 37°C and analyzed for intracellular EGF-R using EGF-R antibody and TSG using anti-HA.