FIG. 5.

Analysis of cold detergent extraction of FasI and GP64 proteins in Sf9 cells and the effect of GP64 palmitoylation. (Top two panels) Infected Sf9 cells expressing both FasI and wild-type GP64 were lysed in 0.5% Triton X-100 at 4, 27, or 37°C. The soluble (S) and insoluble (I) fractions were separated by centrifugation as described in Materials and Methods and subjected to Western blot analysis with either an anti-FasI MAb (top panel) or anti-GP64 MAb AcV5 (second panel). Sf9 cells infected with viruses expressing palmitoylation-minus forms of GP64 (C503A or C503S) were treated as described above, and GP64 was detected with MAb AcV5 (bottom two panels).