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. 2006 Aug 15;398(Pt 2):243–256. doi: 10.1042/BJ20060160

Figure 1. PMA activates Ras in COS-7 cells.

Figure 1

(A) Subconfluent serum-starved COS-7 cells were challenged with 100 nM PMA (TPA). After indicated time periods, cells were lysed in the presence of the Ras binding domain (RBD) of the Ras effector Raf-1 fused to GST and processed for detection of active GTP-loaded Ras as described in the Experimental section. Aliquots of the cell extracts were analysed in parallel to ascertain equal loading. (B) Same set-up as in (A), except that COS-7 cells were stimulated with the indicated concentrations of PMA for 10 min.