Figure 4. U0126 decreases ATP-stimulated extended phase of ATP stimulation of [Ca²⁺]_i.

(A) Cells were plated on to glass coverslips in 35 mm dishes and allowed to attach overnight. Cells incubated in Krebs buffer were inhibited with vehicle or 10 μM U0126 for 15 min at 37 °C before treatment with 10 μM ATP. The results are expressed as the means±S.E.M. for [Ca²⁺]_i above basal (for each 20th data point) from n=5 experiments. (B) Data from each recording were averaged for the net peak [Ca²⁺]_i as well as area under the curve (AUC) for each cell (⁺P<0.05 when compared with ATP treatment alone).