Figure 5. Wild-type and mutant eNOS constructs were evenly expressed within each experiment.

Cells were plated, transfected, and experiments were performed as described for Figures 2 and 3. The strip blots indicate equal protein expression of eNOS between the different constructs transiently expressed by COS-7 cells and investigated in the same experiment (indicated by each solid bar over a group of two to three protein bands). The strip blots represent two of a total of eight blots performed to check for equal eNOS expression. wt, wild-type.