Table 2. U0126 influences ATP-stimulated citrulline production in intact COS-7 cells expressing wild-type and mutant eNOS.

Results for citrulline production are means±S.E.M. for four independent experiments performed in triplicate expressed as percentages of citrulline production after combined U0126/ATP treatment compared with ATP treatment alone. *P<0.05 compared with A23187 treatment alone (100% citrulline production). The far-right column represents the mean±S.E.M. of the ratio of wild-type/mutant citrulline production.

	Citrulline production (%): U0126+ATP/ATP
Construct	Mutant	Wild-type	P value (mutant versus wild-type)	Ratio of wild-type/mutant
S33A	94±6	84±4*	0.31	0.91±0.07
T46A	94±3	86±4*	0.33	0.92±0.07
S58A	92±2*	86±4*	0.30	0.94±0.05
T97A	101±3	88±1*	0.03	0.87±0.02
S872A	93±3	92±2*	0.74	0.99±0.04
S1085A	88±1*	86±4*	0.82	0.99±0.06
T1202A	88±1*	86±4*	0.67	0.98±0.05