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. 2002 Mar;4(2):171–180. doi: 10.1038/sj.neo.7900219

Figure 1.

Figure 1

UCN-01 abrogates both G1 and G2 arrest in p53 proficient cells. Flow cytometric analysis of human HCT116 cells in the absence or presence of 100 nM of UCN-01 and in the absence or presence of 10 Gy, at 6 and 24 hours. G2 arrest at 6 hours was measured as a depletion in M phase cells by a three-dimensional flow cytometry technique, which distinguishes M phase cells based on both DNA content and staining with antibody to mitosis-specific phosphorylated histone H3 (see Materials and Methods section). G2 arrest at 24 hours was measured as an accumulation of cells in G2/M phase through conventional DNA histogram flow cytometry. Cell cycle distributions, shown in upper right of panel, were calculated from the histograms for each irradiation/drug condition.