Table 2.
Summary of Tat activity observed after heterologous expression of tat genes from different bacteria in E. coli tat mutant strains.
E. coli strain | Plasmid-encoded gene(s) | Functionality of the Tat system1 |
JARV16-P(ΔtatA/ΔtatE) | Eco tatA | ++ |
Psy tatA | ++ | |
Sco tatA | ++ | |
Sco tatA(L40F) | ++ | |
Aae tatA1 | + | |
Aae tatA2 | + | |
BØD-P(ΔtatB) | Eco tatB | ++ |
Psy tatB | + | |
Sco tatB | ++ | |
Aae tatB | - | |
B1LK0-P(ΔtatC) | Eco tatC | ++ |
Psy tatC | - | |
Sco tatC | ++ | |
Aae tatC | - | |
DADE-P(ΔtatABCD/ΔtatE) | Eco tatABC | ++ |
Psy tatABC | + | |
Aae tatA1A2tatBC | - |
1++ indicates a high level of functionality (>30% of the wild type level of periplasmic TMAO reductase activity, + indicates a lower level of functionality (<30% of the wild type level of periplasmic TMAO reductase activity, but confers the ability to grow on solid media containing SDS or TMAO), – indicates a lack of activity (no growth on SDS or TMAO-containing plates).