Table 3.
Strains and plasmids used in this study
| Bacterial Strains | Genotype | Source |
| MC4100 | F-ΔlacU169 araD139 rpsL150 relA1 ptsF rbs flbB5301 | [58] |
| DH5α | φ80dlacZΔM15, recA1, endA1, gyrA96, thi-1, hsdR17(rK-,mK+) supE44, relA1, deoR, Δ(lacZYA-argF) U169 | Promega |
| M15 | F-, lac, ara, gal, mtl | [59] |
| JARV16-P | As MC4100 ΔtatAΔtatE pcnB1 zad-981::Tn10d (Kmr) | [8] |
| BØD-P | As MC4100 ΔtatB pcnB1 zad-981::Tn10d (Kmr) | [8] |
| B1LK0-P | As MC4100 ΔtatC pcnB1 zad-981::Tn10d (Kmr) | This work |
| DADE-P | As MC4100 ΔtatABCD ΔtatE pcnB1 zad-981::Tn10d (Kmr) | [60] |
| Plasmids | ||
| pREP4 | KanR, lacI+ | Roche |
| pBluescript (IIKS+) | AmpR | Stratagene |
| pFAT415 | pBluescript carrying E. coli tatA | [8] |
| pFAT416 | pBluescript carrying E. coli tatB | [8] |
| pFAT417 | pBluescript carrying E. coli tatC | [8] |
| pUniprom | AmpR contains E. coli tat promoter and multiple cloning site | [51] |
| pUniprom-PA | pUniprom carrying P. syringae tatA gene | This work |
| pUniprom-PB | pUniprom carrying P. syringae tatB gene | This work |
| pUniprom-PC | pUniprom carrying P. syringae tatC gene | This work |
| pUniprom-PABC | pUniprom carrying P. syringae tatABC genes | This work |
| pUniprom-SA | pUniprom carrying S. coelicolor tatA gene | This work |
| pUniprom-SB | pUniprom carrying S. coelicolor tatB gene | This work |
| pUniprom-SC | pUniprom carrying S. coelicolor tatC gene | This work |
| pFATAQ1 | pBluescript carrying A. aeolicus tatA1and tatA2 | This work |
| pFATAQ2 | pBluescript carrying A. aeolicus tatBC | This work |
| pFATAQ3 | pBluescript carrying A. aeolicus tatA, tatA2 and tatBC | This work |
| pQE70 | C-terminal His-tag expression vector | Qiagen |
| pQEAQ1 | pQE70 carrying A. aeolicus tatA1 | This work |
| pQEAQ2 | pQE70 carrying A. aeolicus tatA2 | This work |
| pQEAQBC | pQE70 carrying A. aeolicus tatBC | This work |
| pQEAQ12 | pQE70 carrying A. aeolicus tatA1and tatA2 | This work |