Figure 3.
Immunocytochemistry for 5-LO pathway enzymes and LT receptors in human bronchial fibroblasts. Representative photomicrographs of fibroblasts from one donor. Fibroblasts were grown to confluence on coverslips and immunostained using primary antibodies against (A) 5-LO, (B) FLAP, (C) LTA4 hydrolase, (D) LTC4 synthase, (E) BLT1 receptors and (F) CysLT1 receptors. Staining with equivalent concentrations of (G) rabbit serum and (H) goat serum are shown as negative controls for A-E and F, respectively. Magnification x400. Positive immunostaining appears red (AEC) against the blue nuclear counterstain (Meyer's haematoxylin).