FIG. 7.
Immunoprecipitation of Env G from infected cell lysates and supernatants. BHK cells were infected with VSV-89.6G (lanes 1), VSV-89.6G(4) (lanes 2), VSV-89.6G(4-7) (lanes 3), or VSV-89.6G(2-7) (lanes 4) for 4 h and labeled with [35S]methionine for 30 min. Immediately after the labeling (time zero) or at 1 or 4 h after the chase, cell lysates and medium were immunoprecipitated with sheep polyclonal anti-gp120 serum. Samples were analyzed by SDS-PAGE (8% acrylamide), followed by exposure to a PhosphorImager screen (Molecular Dynamics). Positions of uncleaved and cleaved Env G are indicated.