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Transposition is temperature sensitive. Several wild-type yeast strains containing a galactose-inducible Ty1 element on plasmid pGTy1m his3AI were quantitated for transposition following galactose induction at the indicated temperatures (as described in Materials and Methods). Strains are as follows: JKc1015 (open circles), W3031-B (solid squares), Hansen BY4741 (solid circles), and PH652 (solid triangles). Full strain genotypes are given in Table 1.
