FIG. 6.

Ty1 cDNA synthesis as a function of temperature. Cells (YH51; Table 1) containing a galactose-inducible Ty1 element were grown at the indicated temperatures. Southern blot analysis of extracted DNA indicates that production of the 3.5-kbp cDNA product is markedly reduced at 30°C and is undetectable at 37°C. Nucleic acids were extracted, digested with EcoRI, treated with RNase, electrophoretically separated (1% agarose gel), and transferred. The membrane was probed with a [³²P]-labeled neo cDNA probe. The larger ∼10.0-kbp band corresponds to the Ty1 plasmid.