FIG. 8.

The PR defect is not reversible. Cells (YH82; Table 1) were induced with galactose overnight at 37°C. Following addition of glucose, the cultures were shifted down to 30°C or maintained at 37°C. Numbers indicate the time points, in hours, at which aliquots were removed. Total cell protein was analyzed by immunoblotting using an antibody to IN. No processed IN was detected.