TABLE 1.
Yeast strains and Ty1 plasmids
| Strain | Genotype | Ty1 plasmida (reference) | Ty1 marker | Strain background |
|---|---|---|---|---|
| YH8 | MATα ura3-167 his3Δ200 leu2Δ1 trp1Δ1 | GRF167 | ||
| YH50 | MATaura3-167 his3Δ200 leu2Δ1 trp1Δ1 spt3-202 | S288C | ||
| YH51 | MATahis4-539 lys2-801 ura3-52 spt3-202 | pJEF1105 (6) | neo | S288C |
| YH82 | MATahis4-539 lys2-801 ura3-52 trp1Δ63 leu2Δ1 | pJEF724 (3) | None | S288C |
| JKc1015 | MATα ura3-167 his3Δ200 leu2Δ1 | pGTy1H3m his3AI (10) | his3AI | GRF167 |
| JKc1031 | MATahis4-539 lys2-801 ura3-52 spt3-202 | pJEF1105 (6) | neo | S288C |
| JKc1032 | MATahis4-539 lys2-801 ura3-52 spt3-202 | pJEF1105PR- (6) | neo | S288C |
| JKc1054 | MATα ura3-167 his3Δ200 leu2Δ1 | pJEF1105 (6) | neo | GRF167 |
| JKc1055 | MATα ura3-167 his3Δ200 leu2Δ1 | pJEF1105PR- (6) | neo | GRF167 |
| W3031-B | MATα ade2-1 can1-100 his3-11,15 leu2-3 trp1-1 ura3-1 | pGTy1H3m his3AI (10) | his3AI | W303 |
| Hansen BY4741 | MATahis3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | pGTy1H3m his3AI (10) | his3AI | S288C |
| YPH652 | MATα ura3-52 his3Δ200 leu2Δ1 | pGTy1H3m his3AI (10) | his3AI | YPH (S288C) |
a
All Ty1 plasmids are GAL-Ty1-H3 URA3 2μm.