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Effect of heparin on PRRSV infection of PAM. PRRSV (▪), PrV (▵), and PrV gCnull (×) were incubated for 1 h at 37°C with different concentrations of heparin. PAM were inoculated with the mixtures, washed after 2 h of incubation at 37°C to remove unbound virus, and finally fixed after 10 h. The data represent the means ± standard deviations (error bars) of triplicate assays.
