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Heparin-dependent kinetics of PRRSV attachment. PAM were inoculated with PRRSV at 4°C, unbound virus was removed by washing the cells after a 5-min incubation at 4°C, and at different time intervals PAM were washed with PBS-F with (▪) or without (□) heparin (2,500 μg/ml). Attachment was evaluated at 4°C by immunofluorescent staining of bound virus particles with streptavidin-FITC and flow cytometric analysis. The data represent the means of duplicate assays.
