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. 2002 May;76(9):4275–4286. doi: 10.1128/JVI.76.9.4275-4286.2002

FIG. 2.

FIG. 2.

Expression of c-myc and bic in CEFs using RCASBP(A)-myc and RCASBP(B)-bic. (a) Schematic of expected RNAs generated from RCASBP(A)-myc and RCASBP(B)-bic constructs. These vectors express cDNA inserts as spliced subgenomic mRNA. SD, splice donor site; SA, splice acceptor site. (b) Northern blot analysis of c-myc and bic expression. Total RNA derived from CEFs mass-infected with RCASBP(A)-myc (lane myc), RCASBP(A) [lane RCAS(A)], RCASBP(B)-bic (lane bic), or RCASBP(B) [lane RCAS(B)] were probed with either a radiolabeled c-myc exon 3 cDNA probe (left) or a bic exon 2 probe (right). The subgenomic mRNA expressing c-myc or bic is indicated by an arrow. The blot was stripped and rehybridized to a chicken actin probe as a control for loading. The radioactive bands observed at the 28S and 18S regions are probably due to hybridization of probe sequence to viral RNAs that comigrated nonspecifically with the rRNAs.