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Localization of altered E1B 55-kDa proteins. HeLa cells infected with Ad5 or the mutant viruses indicated were labeled with [35S]methionine from 13 to 17 h after infection and separated into nuclear and cytoplasmic fractions as described in Materials and Methods. The E1B 55-kDa protein was immunoprecipitated from each sample with monoclonal antibody 2A6.
