FIG. 2.

JEV infection enhances the expression of chaperones. (A) BHK-21 cells treated with 2 μg of A23187/ml (lane A), 2 μg of tunicamycin/ml (lane T), or left untreated (lane M) were harvested 12 h later for Western immunoblotting using antibodies specific for BiP/GRP78, GRP94, calnexin, PDI, and actin as indicated at the left. (B and C) BHK-21 (B) and N18 (C) cells were mock infected (lanes M) or infected with JEV at an MOI of 1, and the cell lysates at the indicated time points (shown above the lanes) were prepared and determined for the expression of chaperone proteins by immunoblotting as described for panel A.