FIG. 6.

Productive infection with JEV did not cause cytotoxicity and UPR in K562 cells. (A) One-step growth curve of JEV and dengue virus serotype 2 (DEN-2) on K562 cells. K562 cells with JEV or DEN-2 (MOI = 5) adsorbed were incubated for various times before their culture supernatants were harvested for plaque-forming assays on BHK-21 cells to determine the virus titers (PFU per milliliter). (B) K562 cell viability after JEV or DEN-2 infection. The numbers of viable mock-, JEV-, or DEN-2-infected K562 cells were determined by trypan blue exclusion 1, 2, or 3 days postinfection. The error bars indicate standard errors. (C) Expression profiles of chaperones and CHOP in JEV-infected K562 cells. K562 cells were mock infected (lane M) or infected with JEV for the indicated periods, and the lysates were harvested for Western immunoblotting assays. The antibodies used in each assay are shown at the right.