FIG. 2.

Cytotoxic T-cell response induced by the synthetic immunogens based on the CTL peptide alone (A) or the CTL-TH construct (B). A ⁵¹Cr release assay was performed with 10⁴ uninfected or 10⁴ Mem 71 virus-infected ⁵¹Cr-labeled P815 target cells and various numbers of effector cells. Secondary effectors were generated from lymph node cells of groups of five BALB/c mice that had been primed 7 days previously with 45 nmol of a peptide immunogen emulsified in CFA. The lymph node cell suspensions were cultured for 5 days with Mem 71 virus-infected autologous spleen cells and then tested in the ⁵¹Cr release assay. Each point on every curve represents the mean of triplicate cultures. For all effector populations, background lysis measured on uninfected P815 targets was <20% at an effector/target ratio of 100:1.