FIG. 6.

Comparison of the number of CTL determinant-specific IFN-γ-secreting cells in lipopeptide- and CTL peptide-primed mice. ELISPOT assays were performed on cells from the inguinal and popliteal lymph nodes of mice primed 7 days previously (left panel) or from the spleens of mice primed 28 days previously (right panel) with the indicated immunogens, emulsified in CFA. The data represent the number of ELISPOTs per total cells recovered, with backgrounds in cultures lacking antigen subtracted. The results are expressed as the mean value obtained from three individual mice, and the error bars represent one standard deviation of the mean.