TABLE 2.
Properties of GALV I264 linker insertion mutant SU proteins
| Mutanta | Location in SU | Processingb | Incorporationc | Infectivityd |
|---|---|---|---|---|
| G47 | N terminus | + | + | − |
| G57 | VRA | + | + | − |
| G77 | VRA | + | + | − |
| G107 | VRA | + | + | − |
| G126 | Between VRA and VRB | + | + | − |
| G149 | Between VRA and VRB | + | + | − |
| G167 | VRB | + | + | − |
| G190 | Between VRB and PRR | + | + | − |
| G219 | PRR | + | + | + |
| G223 | PRR | − | − | − |
| G250 | PRR | + | + | − |
| G315 | C terminus | + | + | − |
| G339 | C terminus | − | − | − |
| G379 | C terminus | + | + | − |
| G439 | C terminus | + | + | − |
a
Linkers were inserted after the residue indicated (e.g., between amino acids 47 and 48 in the first insertion mutant).
b
SU protein was detected (+) or not (−) in lysates from transiently transfected 293T cells, as described in Materials and Methods.
c
SU protein was present (+) or absent (−) in retroviral vector particles produced in 293T cells at 35°C.
d
Vector particles bearing mutant envelopes did (+) or did not (−) infect MDTF-Pit1 target cells.