TABLE 3.
Properties of FeLV-B linker insertion mutant SU proteins
| Mutanta | Location in SU | Processingb | Incorporationc | Infectivityd |
|---|---|---|---|---|
| F47 | N terminus | + | − | − |
| F54 | VRA | + | + | + |
| F72 | VRA | + | − | − |
| F102 | VRA | + | − | − |
| F110 | Between VRA and VRB | − | − | − |
| F137 | Between VRA and VRB | + | + | + |
| F154 | VRB | + | + | + |
| F179 | Between VRB and PRR | + | − | − |
| F210 | PRR | + | + | + |
| F242 | PRR | + | + | + |
| F259 | PRR | + | + | + |
| F359 | C terminus | + | − | − |
| F397 | C terminus | + | − | − |
| F413 | C terminus | + | − | − |
| F422 | C terminus | + | + | + |
a
Linkers were inserted after the residue indicated (e.g., between amino acids 47 and 48 in the first insertion mutant).
b
SU protein was detected (+) or not (−) in 293T cell lysates 48 to 72 h after transfection, as described in Materials and Methods.
c
SU protein was present (+) or absent (−) in retroviral vector particles produced in 293T cells at 35°C.
d
Vector particles bearing mutant envelopes did (+) or did not (−) infect MDTF-Pit1 target cells.