. 2002 May;76(9):4267–4274. doi: 10.1128/JVI.76.9.4267-4274.2002

TABLE 4.

Effect of soluble FeLV or GALV SU on GALV I₁₀ vector infectivity

Vector envelope^a	Target cell	Soluble SU^b	Titer (10⁵ BFU/ml)^c
GALV	MDTF	None	<0.001
	MDTF-Pit1	None	50.0 ± 0.6
GALV I₁₀	MDTF	None	<0.001
	MDTF-Pit1	None	<0.001
GALV I₁₀	MDTF	FeLV-B_1-444	<0.001
	MDTF-Pit1	FeLV-B_1-444	<0.001
GALV I₁₀	MDTF	GALV_1-219	<0.001
	MDTF-Pit1	GALV_1-219	0.8 ± 0.3
GALV I₁₀	MDTF	GALV_1-264	<0.001
	MDTF-Pit1	GALV_1-264	1.2 ± 0.2
GALV I₁₀	MDTF	A-MLV_1-448	<0.001
	MDTF-Pit1	A-MLV_1-448	<0.001

Viral vectors were produced from transiently transfected 293T cells as described in Materials and Methods. GALV I₁₀ contains the C11D8 epitope inserted at position 10 of the SU.

Target cells were incubated with equal volumes of vector particle supernatants and medium containing soluble SU proteins.

Titers are expressed as the average numbers of blue foci (BFU) ± standard errors of the means from three experiments.