Table 1.

Sequences of the PCR primers and internal oligonucleotides probes

PCR product	Primer sequence
Vγ1-Jγ1 circles	Up	5′-Bio-TCCTCAGTGTAAGCCTGTGG-3′
	Down	5′-CCAAACAGCACTTACACTCT-3′
	Internal	5′-CAGAGCAGGTGATCTTCAGAGGGTCCT-3′
Vγ2-Jγ1 circles	Up	5′-Bio-CTTCTGACTGAGCAGCTCAT-3′
	Down	5′-CCAAACAGCACTTACACTCT-3′
	Internal	5′-CAGAGCAGGTGATCTTCAGAGGGTCCT-3′
Vγ3-Jγ1 circles	Up	5′-Bio-TCACAGTAACAGCAAAGTCACC-3′
	Down	5′-CCAAACAGCACTTACACTCT-3′
	Internal	5′-CAGAGCAGGTGATCTTCAGAGGGTCCT-3′
Vβ1-Dβ circles	Up	5′-Bio-GCAGAGAACAAGCAGAAGAC-3′
	Down	5′-ACAGAGCAGTGGCAGAGGAA-3′
	Internal	5′-GTGACTGGATGACAAAATGAATGGAGTGGA-3′
Actin	Up	5′-TACCACAATGTACCCTGGC-3′
	Down	5′-CTCGTCTTGTTTTATGCGC-3′
	Internal	5′-TGGATCAGCAACAGGAGTA-3′
Rag-1	Up	5′-GTGGCAGTTGACTCTTGACA-3′
	Down	5′-GTTCCACTGAATCATTGCTT-3′
	Internal	5′-GCTGTGCCAGTACAGCTATA-3′
Rag-2	Up	5′-GGTACTGGATCATCTGCTGT-3′
	Down	5′-TTCACTGGTGTGGTTAACTT-3′
	Internal	5′-GATCTGTCAGAGAGCATGCT-3′