FIG. 4.

Detection of gH and gL on the cell surface. The detection of gH and gL on the cell surface was optimal when both glycoproteins were coexpressed. CHO cells were transfected with plasmids expressing the indicated HHV-8 glycoproteins. Detection of cell surface (A) and total (B) glycoprotein expression was done by using anti-gH-gL sera as described in the legend to Fig. 3. Shown are the means and standard deviations for three to eight replicate samples. In panel A, the differences between the OD readings obtained for cells transfected with a vector alone and cells transfected with gH or with gH plus gL were both statistically significant (P < 0.001; two-tailed t test), as was the difference between the readings obtained with gH and with gH plus gL (P < 0.001). The difference between the readings obtained with the vector and with gL was not statistically significant (P = 0.774). In panel B, the differences between the readings obtained with the vector and those obtained with gH, gL, or gH plus gL were all statistically significant (P = 0.015, P = 0.007, and P = 0.002, respectively). The difference between the readings obtained with gH and with gH plus gL was not statistically significant (P = 0.632).