FIG. 5.

Cell surface expression of HHV-8 glycoproteins. CHO cells were transfected with plasmids expressing the indicated HHV-8 glycoproteins. After cells were replated on 96-well dishes and incubated for 18 h, they were incubated with primary antibody. Subsequently, cells were fixed and then incubated with biotinylated goat anti-mouse IgG and streptavidin-horseradish peroxidase conjugate. After the addition of substrate, OD readings at 370 nm were obtained. Shown are the means and standard deviations for four replicate samples. In the left panel, the differences between the OD readings obtained for cells transfected with a vector alone and those obtained for cells transfected with gB^MUT alone or with gB^MUT, gH, and gL were both statistically significant (P < 0.001 or P = 0.005, respectively; two-tailed t test), while the differences between readings obtained with the vector and those obtained with gH plus gL or with a combination of gB, gH, and gL were not (P = 1.0). In the right panel, the differences between the readings obtained with the vector and those obtained with gH plus gL, a combination of gB, gH, and gL, or a combination of gB^MUT, gH, and gL were all statistically significant (P < 0.001). The difference between the readings obtained with the vector and with gB^MUT was not statistically significant (P = 0.986).