FIG. 1.

Photomicrographs of immunofluorescence analysis of viral DE antigens expressed at 48 h following infection with Towne and CR208 viruses at a multiplicity of 0.4 PFU/cell. Panels a, c, e, g, i, k, m, o, q, and s are immunostained cells; panels b, f, j, n, r, d, h, l, p, and t are the same respective fields visualized using Hoechst nuclear counterstain; panels a, b, e, f, i, j, m, n, q, and r are HFFs infected with Towne strain HCMV; panels c, d, g, h, k, l, o, p, s, and t are HFFs infected with CR208. Cells were stained for IE2 protein with SMX antibody diluted 1:2 (a and c), ppUL44 DE protein with BS510 antibody diluted 1:25 (e and g), pUL57 DE protein using CH167 antibody diluted 1:10 (i and k), pUL69 DE protein with anti-UL69-66 antibody undiluted (m and o), or pUL98 DE protein using I2 antibody diluted 1:10 (q and s). Color-positive transparencies were digitized with a Nikon Coolscan III slide scanner and converted to grey scale using Adobe Photoshop software. Images obtained of cells stained with the same antibody were exposed and treated identically. Each panel represents a field 150 by 150 μm.