FIG. 5.

Luciferase reporter gene analysis of DE promoter activity during infection by Towne, CR208, and CRQ208 at various multiplicities of infection. HFF, ihf-2, or ihf-ie1.3 cells were transfected with luciferase reporter constructs, infected as indicated, and assayed as described in Materials and Methods. Results from representative experiments with the UL69 promoter (a), UL112-113 and UL44 promoters (b), UL44 promoter (c), UL57 promoter (d), UL54 promoter (e), and UL98 promoter (f) are shown. Data points in panels a, b, and c are the mean ratio of luciferase activation over basal, derived from triplicate data points. Error bars denote a 95% confidence interval for the estimated mean. Data points in panels d, e, and f are the ratio of luciferase activation over basal, obtained in representative single-data point experiments.