TABLE 2.
Reactivity of swine sera and MAbs to selected cloned phages
Phagotope | Reactivitya with primary antibody:
|
||||
---|---|---|---|---|---|
ISU25-C1 | ISU19-A1 | NS 6b | NNS 5c | Swine negative serum | |
Clone 01 (epitope A) ALVNIPISNNLA | 0.045 (± 0.008) | 0.087 (± 0.006) | 0.373 (± 0.016) | 0.855 (± 0.040) | −0.022 (± 0.002) |
Clone 5.1 (epitope B) SHITSYHPAYFW | 0.500 (± 0.003) | 0.067 (± 0.005) | 0.027 (± 0.035) | 0.031 (± 0.018) | 0.037 (± 0.031) |
Clone S2-4 (epitope B) YKNTHLDLIYNA | 0.008 (± 0.001) | 0.039 (± 0.014) | 0.371 (± 0.05) | 0.021 (± 0.020) | 0.045 (± 0.020) |
Results are expressed as corrected OD at 405 nm (OD for selected phage − OD for unselected phage library) (± standard deviation).
NS 6, NS from pig 6 with a neutralizing titer >64 and an ELISA S/P ratio of 1.053.
NNS 5, NNS from pig 5 with a neutralizing titer <4 and an ELISA S/P ratio of 0.652.