TABLE 4.

Reactivity of antipeptide affinity-purified antibodies to synthetic peptides bound to MBS-coated ELISA plates

Peptide	Reactivitya for:
	Eluted R4N-R1N column	Eluted S2 column	Eluted S2 + eluted R1N	Eluted S4 column	Eluted P7b column
S1 (epitopes A and B) VLVNANNSSS SHFQSIYNC	0.164 (± 0.012)	0.269 (± 0.007)	0.436 (± 0.006)	0.007 (± 0.006)	−0.008 (± 0.003)
S2 (epitope A removed, epitope B present) SGSGANNSSSSHFQSIYNC	−0.004 (± 0.004)	0.336 (± 0.005)	0.351 (± 0.025)	0.008 (± 0.000)	0.007 (± 0.024)
S3 (epitopes A and B removed) SGSGANNSSSSGSGSIYNC	0.001 (± 0.002)	0.003 (± 0.004)	0.000 (± 0.001)	0.002 (± 0.001)	−0.009 (± 0.005)
S4 (mimotope B) SHITSYHPAYFWC	−0.007 (± 0.001)	0.010 (± 0.021)	NDc	−0.005 (± 0.005)	−0.010 (± 0.003)
R4N (mimotope A) ALVNIPISNNLAC	0.540 (± 0.027)	0.002 (± 0.002)	ND	0.010 (± 0.006)	0.015 (± 0.007)
R1N (epitope A) VLVNSNNSSSSAC	0.410 (± 0.024)	0.000 (± 0.005)	ND	0.009 (± 0.001)	0.005 (± 0.019)
Δ4R1N (epitope A removed) SGSGSN NSSSSAC	0.005 (± 0.008)	0.001 (± 0.003)	ND	0.004 (± 0.001)	0.004 (± 0.009)

^aResults are expressed as corrected OD at 405 nm (OD for tested peptide − OD for irrelevant peptide P7) (± standard deviation).

^bIrrelevant peptide P7 (QRAYLELPPWPP).

^cND, not done.