TABLE 5.
Specific recognition, by two sequential serum samples from six HI pigs, of epitopes A and B
| Epitope | Recognitiona at indicated timeb by sample from pig:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1
|
2
|
3
|
4
|
5
|
8
|
|||||||
| A (1.432, 0) | B (1.755, 32) | A (1.266, 4) | B (1.998, 64) | A (1.078, 0) | B (2.030, 512) | A (1.382, 4) | B (1.993, 128) | A (0.652, 0) | B (1.29, 64) | A (0.946, 0) | B (1.712, 128) | |
| A (clone S2-4) | 0.019 (± 0.020) | 0.072 (± 0.016) | 0.033 (± 0.004) | 0.170 (± 0.011) | 0.014 (± 0.011) | 0.155 (± 0.005) | 0.000 (± 0.027) | 0.493 (± 0.012) | 0.021 (± 0.004) | 0.062 (± 0.001) | 0.044 (± 0.015) | 0.249 (± 0.004) |
| B (clone 01) | 0.738 (± 0.018) | 0.289 (± 0.019) | 0.782 (± 0.039) | 0.237 (± 0.019) | 0.704 (± 0.069) | 0.594 (± 0.037) | 0.689 (± 0.019) | 0.755 (± 0.017) | 0.855 (± 0.04) | 0.431 (± 0.019) | 0.828 (± 0.008) | 0.231 (± 0.005) |
a
Recognition is expressed as corrected OD at 405 nm (OD for phage clone − OD for unselected phage library) (± standard deviation).
b
Times A and B correspond to early (20 days p.i.) and advanced (7 months p.i.) stages of the hyperimmunization protocol, respectively. Values in parentheses are ELISA S/P ratios, determined as indicated in Materials and Methods, followed by SN titers.