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. 2006 Apr 6;35(3):378–386. doi: 10.1165/rcmb.2006-0027OC

Figure 1.

Figure 1.

Figure 1.

Figure 1.

Figure 1.

Adhesion of purified blood and airway EOS to diverse integrin ligands. Adhesion of purified blood EOS of unchallenged subjects (A), blood EOS of challenged subjects (B), or airway EOS obtained after antigen challenge (C) to diverse integrin ligands coated at 10 μg/ml or with a solution of 1% BSA and blocked with FBS. Results are the mean adhesion from six separate donors performed in triplicate wells on six different occasions (18 wells) with the SEM indicated. *P < 0.001, comparing adhesion to ligands versus the FBS blocker; one-way ANOVA with Dunnett's post test. P < 0.001, §P < 0.05, comparing adhesion of airway EOS to blood EOS of unchallenged or challenged subjects; one-way ANOVA with Dunnett's post test. (D) Specific adhesion of blood EOS of unchallenged and challenged subjects and airway EOS of challenged subjects to increasing coatings of VCAM-1. Specific adhesion to VCAM-1 was calculated by subtracting nonspecific adhesion to FBS blocker from adhesion to VCAM-1. Results are the mean from five separate donors performed in triplicate wells on five different occasions (15 wells) with the SEM indicated. COL, collagen type I; FG, fibrinogen; FN, fibronectin; LN, laminin; VN, vitronectin.