TABLE 1.

ACTIVATION STATES OF INTEGRINS OF PURIFIED EOS

	Mean Specific gMCF* ± SD
	Activated αM		Activated β1
	CBRM1/5	N29	HUTS-21	9EG7
Unchallenged blood EOS	< 110 (7)	140 ± 130 (9)	< 110 (6)	< 110 (6)
Challenged blood EOS	150 ± 120 (5)	170 ± 110 (6)	< 110 (6)	< 110 (5)
Challenged airway EOS	460 ± 250† (5)	< 110 (8)	< 110 (4)	< 110 (4)

Definition of abbreviations: EOS, eosinophils; gMCF, geometric mean channel fluorescence.

Results from different donors for purified EOS are shown.

^*Detected with FITC-conjugated secondary mAb; data are shown as the mean specific gMCF in assays performed in HBSS buffer with standard deviations indicated. Epitopes exhibiting a specific gMCF less than 110 are counted as not recognized.

^†Reactivity is significantly different from Purified Unchallenged and Challenged Blood EOS, P < 0.001, repeated measures ANOVA.