Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Replication of HAV in MO/MAC cultures. MO/MAC were isolated from two different buffy coat samples by plastic adherence [BC(A), —· ·•· ·—; BC(B), —· ·▪· ·—] and infected with HAV (MOI = 1). At the times indicated, the level of infectious HAV was determined by titration of the cell lysates in HFS-10 cells followed by HAV-specific immunofluorescence microscopy. The TCID50 was calculated according to the method of Kärber (11a).
