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VP4, the spike protein of rotavirus, is present in the DRM fraction. Caco-2 cells infected with rotavirus (10 PFU/cell) were treated with 1% Triton X-100 on ice 18 h p.i., and DRM were floated on a sucrose gradient as described in Materials and Methods. Twelve fractions were collected, fraction 1 representing the top of the gradient. Aliquots of each fraction were precipitated with TCA, and the distribution of rotavirus VP4 and AP along the gradient was assayed by immunoblotting.
