FIG. 9.

Influence of virus particles on the phase behavior of a model lipid mixture. The lipid mixture used in these experiments contained phosphatidylethanolamine-phosphatidylserine-sphingomyelin-cholesterol (4/1/2/2 [mol/mol]). Lipids were fully hydrated in the presence of calcium (1 cation/1,000 lipids). (A) The quaternary mixture retains a single inverted hexagonal phase between 14 and 45°C (heating temperature scan rate, 2°C/min). The diffraction peaks point to the repeat distance (d) of 6.23 nm, with a second-order signal corresponding to the periodicity d:d/1:d/2: … . (B) When viral particles are added to the previous lipid mixture, the phase is changed to a single lamellar arrangement characterized by a repeat distance of 6.77 nm (6.77/3.41 for the periodicity 1/[1/2]).