Table 3.
Development of embryos after microinsemination of oocytes with spermatozoa and spermatids from frozen epididymides and testes, transported internationally (B6 male germ cells and B6 oocytes)
| Male germ cells | Cells suspension medium | No. of two-cell embryos transferred | No. of recipient females |
Total no. of implantation sites (%) | No. of normal term fetuses (%) | |
|---|---|---|---|---|---|---|
| Total | Pregnant | |||||
| Epididymal sperm | GL-PBS | 63 | 5 | 0 | 0 (0.0)* | 0 (0.0)‡ |
| NIM | 38 | 3 | 2 | 17 (44.7)** | 9 (23.7)‡‡ | |
| Testicular sperm | GL-PBS | 50 | 4 | 4 | 34 (68.0)† | 11 (22.0)§ |
| NIM | 20 | 2 | 2 | 19 (95.0)†† | 13 (65.0)§§ | |
| Testicular elongated spermatids | GL-PBS | 10 | 1 | 1 | 3 (30.0) | 1 (10.0) |
| NIM | 39 | 4 | 3 | 12 (30.8) | 7 (17.9) | |
∗, ∗∗, P < 5 × 10−8. †, ††, P < 0.05. ‡, ‡‡, P < 1 × 10−4. §, §§, P < 1 × 10−3 (Fisher’s exact probability test). ‡, ‡‡, P < 1 × 10−4. §, §§, P < 1 × 10−3 (Fisher’s exact probability test).