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Presence of heat shock proteins in HIV-1 virions. Virions produced by 293T cells transfected with HIV-1NL4-3 proviral DNA were purified by centrifugation through a 25% sucrose cushion. The purified virions were either mock treated (lane 2) or treated with 0.2 mg of subtilisin/ml (lane 3). Subsequently, the transfected cell lysates (lane 1) and virions (lanes 2 and 3) were analyzed by Western blotting with antibodies against gp41, HIV-1 CA, and the indicated heat shock proteins.
