Figure 4.

Mapping of the BRCA1 binding domain on CBP. (A) Pull-downs with the indicated CBP amino acids fused to GST and an extract from 293 cells overexpressing full-length BRCA1. Approximately 10% of the input is shown in lane 7 of the anti-BRCA1-AbD Western blot. The arrow points to full-length BRCA1. (B) GST pull-down of in vitro-translated ³⁵S-labeled BRCA1 using GST or the GST-CBP 451–721 fragment. Pulled-down complexes were resolved by SDS/PAGE and visualized by autoradiography. (C) CBP/p300 domain structure with some of the known interaction partners.