FIG.2.

Morphologies of viral inclusions in T1L- and T3D^N-infected cells detected by immunostaining for other viral proteins. T1L- or T3D^N-infected CV-1 cells were fixed and immunostained at 18 h p.i. (A) The subcellular localizations of μNS (left column) and λ2 (upper right column) were detected by immunostaining with rabbit anti-μNS serum and a mouse monoclonal antibody to λ2 (57), followed by Alexa 594-conjugated goat anti-rabbit IgG and Alexa 488-conjugated goat anti-mouse IgG. The subcellular localizations of μNS (left column) and μ2 (lower right column) were detected by immunostaining first with rabbit anti-μ2 serum followed by Alexa 488-conjugated goat anti-rabbit IgG (green) and then with rabbit IgG that was purified from the anti-μNS serum and then conjugated with Texas Red (red). Scale bars, 10 μM. (B) Higher magnification of the codistribution of μNS and μ2 in T1L- and T3D^N-infected cells. Merged images are shown in the right column: μ2 (green) and μNS (red). Arrows indicate localized accumulations of μ2.