FIG. 7.

Hyperacetylation of MTs in T1L- and T3D^C-infected, but not T3D^N-infected, CV-1 cells. (A) CV-1 cells were infected with T1L, T3D^C, or T3D^N at an MOI of 5 and then fixed 18 h p.i. in 100% methanol. The cells were immunostained with rabbit anti-μNS serum and then with Alexa 594-conjugated goat anti-rabbit IgG (red) together with a mouse monoclonal antibody to acetylated α-tubulin (47, 48), followed by Alexa 488-conjugated goat anti-mouse IgG (green). The nuclei were counterstained with DAPI (blue). Scale bars, 10 μm. (B) Cells in 60-mm-diameter dishes were mock infected or infected with T1L, T3D^C, or T3D^N at an MOI of 5, and lysates were collected 18 h later. The lysates were subjected to electrophoresis on 10% polyacrylamide gels and electroblotting to nitrocellulose for immunodetection of acetylated α-tubulin (ac. tubulin) and α-tubulin (tubulin).