TABLE 1.
Primers used to make mutations of serine at position 389 to alanine, glycine, aspartic acid, asparagine, glutamic acid, and glutamine on rabies virus N
| Primer | Primer sequencea (5′-3′) | Amino acid resulting from change |
|---|---|---|
| SA5 | GATGATGGAACTGTCAACGCTGACGACGAGG | Alanine |
| SA3 | GTAGTCCTCGTCGTCAGCGTTGACAGTTCC | |
| SG5 | GATGATGGAACTGTCAACGGTGACGACGAGG | Glycine |
| SG3 | GTAGTCCTCGTCGTCACCGTTGACAGTTCC | |
| SD5 | GATGATGGAACTGTCAAGATGACGACGAGG | Aspartic acid |
| SD3 | GTAGTCCTCGTCGTCATCGTTGACAGTTCC | |
| SN5 | GATGATGGAACTGTCAACAATGACGACGAGG | Asparagine |
| SN3 | GTAGTCCTCGTCGTCATTGTTGACAGTTCC | |
| SE5 | GATGATGGAACTGTCAACGAAGACGACGAGG | Glutamic acid |
| SE3 | GTAGTCCTCGTCGTCTTCGTTGACAGTTCC | |
| SQ5 | GATGATGGAACTGTCAACCAAGACGACGAGG | Glutamine |
| SQ3 | GTAGTCCTCGTCGTCTTGGTTGACAGTTCC |
a
Mutated codon is in boldface.