TABLE 1.
Clinical signs following infection of ferrets with HK/483 or HK/486 H5N1 viruses
| Day p.i. | n | No. of ferrets with clinical signs
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Decreased activitya
|
Inappetenceb
|
Nasal dischargec
|
Sneezing
|
Dyspnead
|
Diarrhea
|
Conjunctival dischargee
|
Neurologic signsf
|
||||||||||
| 483 | 486 | 483 | 486 | 483 | 486 | 483 | 486 | 483 | 486 | 483 | 486 | 483 | 486 | 483 | 486 | ||
| 1 | 9 | -g | - | 1 | - | 4 | - | - | - | - | - | 1 | - | - | - | - | - |
| 2 | 7 | 5 | 3 | - | - | 3 | 2 | - | 1 | - | - | 2 | - | 1 | - | - | - |
| 3 | 7 | 7 | 6 | 2 | 1 | 3 | 2 | 1 | - | - | - | 3 | - | - | - | - | - |
| 4 | 5 | 5 | 5 | - | - | 2 | 1 | - | 3 | 1 | - | 3 | 1 | - | - | - | - |
| 5 | 5 | 4 | 5 | 2 | - | 3 | 2 | 1 | - | - | - | - | 1 | 1 | - | - | - |
| 6 | 3 | 2 | 3 | - | - | 1 | - | - | - | - | - | - | - | - | - | - | - |
| 7 | 3 | 2 | 3 | - | - | - | - | 2 | - | - | - | - | - | - | - | - | - |
| 8 | 3 | 2 | 3 | - | - | 1 | - | - | - | - | - | 1 | - | - | - | - | - |
| 9 | 3 | - | 3 | - | - | - | - | - | - | - | 1 | - | 1 | - | - | - | - |
| 10 | 3 | - | 2 | - | 3 | - | - | - | - | - | - | - | - | - | 1 | - | 1 |
| 11 | 3 | - | 3 | - | 2 | - | 1 | - | 1 | - | - | - | - | - | 1 | - | 2 |
| 12 | 3 | - | 3 | - | 3 | - | 1 | - | - | - | - | - | - | - | 1 | - | 2 |
| 13 | 3 | - | 3 | - | 3 | - | 1 | - | - | - | - | - | - | - | 1 | - | 2 |
| 14 | 3 | - | 3 | - | 3 | - | 1 | - | - | - | - | - | - | - | 1 | - | 2 |
Number of animals with activity scores of ≥1.0 determined as described in Materials and Methods.
Decreased appetite was based on absence of food in the stomach at necropsy or failure to remove food from the food hopper.
HK/483-infected ferrets developed a serous nasal discharge, while HK/486-infected ferrets developed a mucopurulent nasal discharge.
Ferrets exhibited open-mouth breathing with exaggerated abdominal movement. Aural assessment by investigator was not possible due to the use of a personal air-powered respirator.
HK/483-infected ferrets developed a serous ocular discharge, while HK/486-infected ferrets developed a mucopurulent ocular discharge.
Neurological signs were hind-limb paresis, ataxia and torticollis. In another experiment, two ferrets per group were infected with 104, 103, or 102 EID50 (equal to 100, 10, and 1 FID50, respectively) of HK/483 or HK/486. On day 7 p.i., one of the two ferrets infected with each dose of HK/483 exhibited the aforementioned neurological signs. One of two ferrets infected with 103 or 102 EID50 of HK/486 neurological signs on days 8 and 13 p.i., respectively. In a third experiment, three of four ferrets infected i.n. with 107 EID50 (105 FID50) of HK/486 exhibited neurological signs.
-, No animals had a particular clinical sign.