FIG. 1.

(A) The first schematic represents the secreted gp120 form of the envelope used as a vaccine insert. The second schematic represents the sgp120–C3d₃ construct used as a vaccine insert. The third schematic represents the C3d₃ construct used as a vaccine insert. Linkers composed of two repeats of four glycines and a serine {(G₄S)₂} were fused at the junctures between gp120 and C3d and between each C3d repeat. (B) V3 loop sequence representing R2, ADA, and 89.6. The superscript Pro–Met represents proline and methionine mutation introduced into gp120_ADA and gp120_89.6. Undelined amino acids indicate additional amino acids that differed with the R2 sequence.