Table 2.

Binding of ¹²⁵I Radiolabeled HIV-1 Envelope to HOS Cells Expressing the Coreceptors hCCR5 or hCXCR4

	Coreceptor expressing cellsa
Samples	hCCR5	hCXCR4	Anti-hCCR5b
R2
wt gp120	22,501 ± 3,510c	355 ± 127	174 ± 57
wt gp120–mC3d3	22,013 ± 1,568	241 ± 205	256 ± 32
co gp120	17,129 ± 3,629	267 ± 130	50 ± 29
co gp120–mC3d3	18,219 ± 578	97 ± 75	117 ± 92
ADA
wt gp120	323 ± 510	200 ± 110	152 ± 71
wt gp120–mC3d3	287 ± 168	41 ± 23	129 ± 86
co gp120	297 ± 62	67 ± 130	96 ± 92
co gp120–mC3d3	253 ± 57	97 ± 75	117 ± 41
ADA (PM)
wt gp120	19,445 ± 2,520	65 ± 12	74 ± 17
wt gp120–mC3d3	15,553 ± 1,678	79 ± 135	67 ± 44
co gp120	23,894 ± 2,004	234 ± 108	95 ± 20
co gp120–mC3d3	16,283 ± 1,741	197 ± 107	150 ± 37
89.6
wt gp120	384 ± 139	105 ± 62	113 ± 30
wt gp120–mC3d3	446 ± 43	333 ± 143	129 ± 43
89.6 (PM)
wt gp120	21,490 ± 3,102	17,265 ± 1,009	372 ± 170
wt gp120–mC3d3	29,835 ± 4,688	9,312 ± 4,510	174 ± 74

^aHOS cells expressed either hCCR5 or hCXCR4, but not hCD4

^b10 mg/ml of 17b mixed with protein prior to cell incubation

^cValue represents the inverse of the endpoint dilution titer. All values represent the average counts per minute of three independent experiments ± SEM